Extraction and Characterization of Protease From Senesced Leaves of Papaya (Carica Papaya) and It’s Application

Natural senescence is associated with several fold increase in activity of protease and is characterized by the degradation and loss of proteins therefore plant proteases have been a common studied interestingly. Protease from senesced leaves of the papaya plant (Carica Papaya) was partially purified by ammonium sulfate precipitation. It showed 15 fold purification. Optimum protease activity was found at alkaline range 9 and 60°C. Mn ion had the least effect on the activity of the enzyme. While Cu showed the maximum inhibitory effect on the protease activity. The enzyme was then used in further application in the detergent industry. We reported that the enzyme remained active and can be used to destain protein stains (blood, leaf extract, flower pigment and turmeric) from prestained cloth. The easy availability of the senesced leaves of this common plant makes it a cheaper enzyme source and potential additive in detergents.